<p>The extracted oil gave a yield of 18.6%. Two of the three identified bacterial strains were susceptible with diameters of 17mm for Salmonella typhi and 18,5mm for Salmonella paratyphi A. Their minimum inhibitory and bactericidal concentration were 37.5mg/ml and 150mg/ml respectively with a MBC/MIC ratio equals to 4; which demonstrates that, oil from the Azadirachta indica seeds used had anti-salmonella bacteriostatic properties. Acute toxicity analyses showed that the lethal dose 50 (LD 50) of this oil was greater than 2ml/100g.</p>