<p>We conducted a descriptive cross-sectional study. A case was any patient admitted to the NICU with A. baumannii cultured from blood or cerebrospinal fluid (CSF) between 01 January and 16 August 2022. Diagnostic laboratory data from the NICU were analyzed. We assessed the clinical characteristics of the five neonates with reported infections and performed whole genome sequencing (WGS) to determine the genetic relatedness of the isolates. An observational assessment of the NICU was conducted on 18 August 2022 to investigate possible sources of MDR-AB transmission.</p>