<p>A cross-sectional study was conducted from January to April 2022 among HIV-infected women attending PMTCT clinic at Bukoba Municipality. Clean-catch midstream urine specimens were cultured on MacConkey and blood agars. Bacteria isolated were identified by colonial characteristics, Gram stain and biochemical identification tests. After initial screening, double-disc synergy method confirmed the phenotypic detection of ESBL production and MRSA was confirmed using cefoxitin disc (30μg). Socio-demographic, clinical, and laboratory information data were collected using a structured questionnaire. STATA version 15.0 was used for analysis. Test for association was performed using modified Poisson regressions.</p>