<p>The prevalence of folic acid deficiency (0.05). However, mean serum folic acid, vitamin B12, vitamin D, and calcium of pregnant women not using corresponding micronutrient supplements (5.29 ± 0.20 ng/mL, 454.64 ± 26.35 pg/mL, 31.75 ± 0.70 ng/mL, 9.03 ± 0.04 respectively) was significantly lower than that of pregnant women using the corresponding micronutrient supplements (7.55 ± 0.22 ng/mL, 640.48 ± 26.38 pg/mL, 36.09 ± 0.78 ng/mL, 10.04 ± 0.22 respectively) (p&lt;0.05). Only 38% of pregnant women and 27% of non-pregnant women have good knowledge on folic acid. Good practice was however noticed in about 69% of pregnant women and 77% of non-pregnant. Six percent (6%) of the subjects indicated that they had history of child with NTD in the family. Serum folic acid, vitamin B12, and calcium levels of those with history of NTD (mean = 7.53 ± 0.52 ng/mL, 664.71 ± 58.84 pg/mL, and 9.71 ± 0.04 mg/dL respectively) was significantly higher compared to that of those with no history of NTD (mean = 6.23 ± 0.12 ng/mL, 524.10 ± 13.68 pg/mL, and 9.12 ± 0.04 mg/dL respectively). Of all subjects, only 18% generally plan on when to be pregnant and about 30% have had one or more abortion.</p>