<p>Cholera remains a global threat to public health and an indicator of inequity and lack of social development. On February 22, 2023, South Sudan Ministry of Health was alerted of a suspected cholera outbreak in Malakal County after two patients with acute watery diarrhoea (AWD) tested positive for Vibrio cholerae using rapid diagnostic tests (RDTs). We conducted further investigations to confirm the outbreak, characterize and identify risk factors to inform control and prevention measures.</p>