<p>Breast milk provides the optimal nutritional needs for growing infants, yet only 66% of the children less than six months receive breast milk which is below the 70% WHO target. Additionally, infant mortality rate is estimated at 33.8 per 1000 live births. Despite research indicating that breast milk can significantly reduce infant morbidity and mortality, it has not been practiced due to various challenges like preterm birth where mothers are not able to produce breast milk immediately. The act of donating breast milk, provides an opportunity for optimal health to infants who unfortunately have no access to breast milk. However, there is a knowledge gap on the awareness and acceptability of breast milk donation and its associated factors among mothers in Kampala District. The objective was to determine awareness and acceptability of human breast milk donation and its associated factors among mothers in Kampala District.</p>