<p>We carried-out a cross-sectional study in 6 health facilities in Mukono district, namely; Mukono general hospital, Goma HCIII, Kojja HCIV, Seeta-Nazigo HCIII, Kasawo HCIII and Nakifuma HCIII. A mother was defined as a woman in her prenatal period or having delivered six months prior to the interview. We interviewed 348 mothers on newborn care using Interviewer administered questionnaires. Descriptive analysis was used to determine maternal knowledge level of newborn care and their practices. Using STATA v.14.0, Prevalence ratios at multivariate analysis were used to determine factors associated with knowledge and practice of newborn care among mothers.</p>