<p>Serum antibodies against JEV were detected in wild boars (92.2%, 189/227), sika deers (7.3%, 15/158) and Nutria (0.5%, 1/61). All the badgers (n=9) and Japanese macaques (n=21) tested negative. In the final multivariable logistic regression model, the odds of testing seropositive were higher for wild boars (OR=365.39, 95%CI 41.63- 3205.49) and Sika deers (OR=2.27. 95%CI 0.22, 23.29) when compared to Badger, Nutria and Japanese macaque. Compared with Mine city, the odds of testing seropositive were lower for Sanyo-Onoda city (OR= 0.27, 95% CI 0.11, 0.69) but similar to that of Ube city (OR= 0.29, 95%CI 0.08, 1.05) and Yamaguchi city (OR= 0.40, 95% CI 0.10, 1.59). Also, the odds of testing seropositive were higher for samples collected in the years 2021 (OR= 5.72, 95%CI 2.52, 12.97) and ≥ 2022 (OR=2.32, 95%CI 1.07, 5.03) compared with ≤2020.</p>