<p>Antimicrobial resistance (AMR) is a major urgent threat to clinical medicine and public health systems. An estimated 10 million death in 2050 will be due to antibiotic resistance and 90% of these deaths will be in low- and middle-income countries. In a country with poor resources, such as Yemen, AMR can cause an increase in mortality and morbidity due to treatment failures and a lack of effective therapy. The aims are to describe the epidemiological bacteria and multi-drug resistance of Gram-positive bacteria and Gram-negative bacteria isolated at main laboratories in Sana'a city.</p>