<p>A case was defined as a pig that had aborted or exhibited any reproductive disorder on the farms within its reproductive period. Blood samples and vaginal swabs were obtained from all the sows and gilts from the affected farms (Farm A and B). Abortion history and husbandry practices, use of personal protective equipment (PPE), were assessed using a structured questionnaire. A Multiplex High-Resolution Melting (HRM) based real-time PCR assay targeting Brucella spp., Leptospira spp., Coxiella burnetii and Listeria monocytogenes was done</p>