<p>The study was a retrospective cross-sectional study design, and the sampling type was consecutive sampling including all children under 5 years of age with acute diarrhea who were registered in the study period were included in this study. Data were analysed by using Statistical Package for Social Sciences (SPSS) version 26. A total of 4,239 children presenting with acute watery diarrhea visited at Cholera Treatment Center (CTC) were recruited from June 2022 to July 2023. a stool sample specimen was collected from enrolled child, which was tested for rotavirus antigen using enzyme-linked immunosorbent assay (ELISA ProSpecT® Rotavirus Microplate Assay), for detection of rotavirus in fecal specimens.</p>