Of the 290 study participants, 66 (22.8%) had significant bacteriuria. The predominant bacteria isolated were E. coli 21 (31.8%). 17 (34.0%) of gram-negative bacteria were Extended Spectrum Beta Lactamase (ESBL) procedures and 1 (25.0%) of Staphylococcus aureus were Methicillin-resistant Staphylococcus aureus (MRSA). Escherichia coli showed high rate of resistance rate against trimethoprim-sulfamethoxazole 21(100%), amoxicillin/clavulanic acid 20 (95.0%). Staphylococcus aureus were resistance to penicillin 4 (100%) and trimethoprim-sulfamethoxazole 4 (100%). The proportion of multidrug resistance (MDR) was 45 (68.2%). Rwamishenye health Centre (APR: 2.12, 95%CL: 1.04-4.34, p=0.027), Kashai dispensary (APR: 2.63, 95%CL: 1.31-5.26, p=0.006) and single marital status (APR=1.85, 95%CI:1.03-3.34) were significantly associated with bacteriuria.