Methods: We conducted a retrospective descriptive analysis of diarrhea cases extracted from worksheets at Athlone hospital laboratory, Lobatse (AHLL).  The sample were children aged 0-59 months seen at district health facilities whose stool samples were obtained and analyzed at AHLL between January 2021 to August 2022. Samples were cultured for Adenovirus, Rotavirus, Salmonella, Shigella, and Escherichia coli species. We compared the number of samples analyzed to the number of diarrhea cases reported in the district and examined the completeness of data. We calculated frequencies and proportions for samples that tested positive to any pathogen, and samples that tested positive for each pathogen. We described seasonality of diarrhea and turnaround time for samples.