We conducted analytical cross-sectional study among 106 randomly sampled staff and students at the School of Public Health, University of Ghana, Accra, on the 29th of April 2022. A pretested paper-based structured questionnaire and observational checklist were used to assess knowledge, attitude, and hand hygiene practices. A category score of &amp;gt;60% was considered good while &amp;lt;60%, poor. Data was summarized into frequencies and proportions using STATA version 16. Logistic regression at a p-value of &amp;lt;0.05 was used to determine associations. 