In 2019, approximately 10.4 million people suffered from Tuberculosis (TB) globally, and in Kenya, it was 558/100,000 population. Directly observed treatment for TB, short-course (DOTs) ensures people suffering from TB have equitable access to high-quality diagnosis, treatment, care, and prevention. However, factors associated with its use in Mombasa County are unknown. We sought to determine factors associated with the use of DOTs among tuberculosis patients in Mombasa County.