We conducted a cross-sectional study of TB and COVID-19 testing at University Teaching Hospital, in Lusaka, Zambia. We linked TB and COVID-19 polymerase chain reaction (PCR) laboratory results from August 2020 to June 2022 using probabilistic matching based on name and age. Testing was done based on clinician suspicion of TB and/or COVID-19 but no information on clinical symptoms was available. We defined TB-COVID-19 coinfection as the occurrence of a positive PCR test for both infections within one month. We calculated the proportion of TB-COVID-19 coinfection among those tested to determine the prevalence and conducted Pearson’s Chi-square test to determine the association between TB and COVID-19 infection.