Antibiotics are powerful medicine that are mostly used to fight certain infections, and if not properly used can have severe and direct consequence on public health. Antibiotics are used in livestock farming for meta-prophylaxis, growth promoters and therapeutically to maintain health, these factors can lead to antimicrobial resistance. This study was carried out to assess the knowledge, attitude and practice of animal health professionals in the 21 local government areas of Adamawa State, northeastern Nigeria, and determine factors that could influence the use and misuse of antibiotics.