Continuous monitoring of antimicrobial resistance (AMR) among isolates from clinical samples can inform effective drug selection for patients. In Uganda, human AMR surveillance occurs at national and regional referral hospitals and in selected public universities. Bacterial isolates from patients are subjected to drug susceptibility testing; the results are used in real-time for patient care. Isolates are then sent to the National Microbiology Reference Laboratory (NMRL) for re-analysis to generate national AMR surveillance data and for global reporting. Although isolate analysis results from NMRL are considered the official AMR surveillance data, there is limited utilization of this data to inform public health planning. We evaluated trends and spatial distribution of AMR to common antibiotics used in Uganda.