Introduction of measles vaccines led to a 73% decreased mortality between 2000 and 2018 globally. However, about 169,000,000 children missed the first dose, leading to an estimated 140,000 deaths in 2018 worldwide. In Ghana, Atebubu-Amantin District has been experiencing focal measles outbreaks since 2016 and a major outbreak in 2018. Given that measles herd immunity is achieved as 95% vaccination coverage, we conducted this study to determine measles vaccination coverage (MVC) and factors influencing vaccination uptake in the district.