A cross-sectional study design was used. A total of 475 HCWs were recruited using systematic random sampling method from twelve health facilities in Tigray, Ethiopia. Eligibility criteria included those HCWs who were 18 years old, permanent employee, and on duty during the study period. A pre-tested structured questionnaire was developed and administered by interviewer. Knowledge, perception and attitudes on COVID-19 were measured through 19 and 12 question items, respectively. Descriptive and binary logistic regression analysis were conducted using SPSSS version 23.