: Novel strains of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) harboring di-verse gene mutations have periodically emerged and are spreading globally. Importantly, mutations within the spike gene's receptor-binding domain (RBD) are linked to SARS-CoV-2 transmissibility, disease severity, or decreased diagnostic or vaccine efficacy. To identify SARS-CoV-2 variants rapidly, we used a single primer-based PCR method in conjunction with Sanger sequencing. 