This study was conducted as part of the World Health Organization (WHO) supported rotavirus diarrhea disease surveillance. Stool samples were collected from &amp;lt;5-year-old children with diarrhea following World Health Organization criteria at the sentinel site, the Complexe Pédiatrique hospital in Bangui. The samples were first screened for group A rotavirus antigen by enzyme immunoassay (EIA) using ProSpecT™ Rotavirus Microplate Assay. Ten percent (10%) of the stool received at Institut Pasteur de Bangui were used for quality control and RNA extracted from EIA-positive samples were subjected to semi nested reverse-transcriptase polymerase chain reaction (RT-PCR). 