Hepatitis B Virus (HBV) infection affects the liver and can result in acute or chronic liver disease. HBV is transmitted by exposure to infectious blood and other body fluids and healthcare workers (HCWs) are more likely to acquire infection compared to general population. Although an effective vaccine is available for the prevention of HBV,  its uptake among HCW may be hindered by misconceptions and attitudes among HCW, cost and health system challenges. We assessed proportions of HCW vaccinated for HBV and factors affecting vaccination uptake in Othoro, Ober and Kabondo Level IV hospitals in  Homabay county Kenya.