<p>Using the microdilution assay, the antibacterial activity of Azadirachta indica seed oil was evaluated on three Salmonella strains. Subsequently, an acute toxicity study of the extract was performed on Wistar rats by dosing the test groups with 2 ml/100kg of body weight, according to the slightly modified Organisation for Economic Co-operation and Development (OECD) guideline 420.</p>