<p>Cholera remains a global public health threat and an indicator of inequity and lack of social development. On 14th April 2022, South Sudan National Public Health Laboratory (NPHL) isolated Vibrio cholerae from stool sample of an internally displaced person (IDP) in Bentiu Camp, Rubkona County, Unity State, following unprecedented floods. We investigated to characterize the outbreak, determine the magnitude, identify the source and implement control and prevention measures.</p>