<p>HIV testing services (HTS) are crucial to achieving the 2025 UNAIDS global 95-95-95 targets. In Uganda in 2020, only 81% of persons living with HIV (PLHIV) knew their status, falling short of the first 95%. Routine HIV testing yielded only 2.7% positive during 2022. Assisted partner notification (APN), a targeted HIV testing method introduced in 2017, aims to identify PLHIV with unknown status using minimal resources by interviewing known HIV-infected persons about sexual partners and confidentially offering those partners HTS. We examined trends and distribution of HIV testing yield among partners aged ≥15 years in Uganda during 2020-2022.</p>