<p>New HIV infections continue among adolescent girls and young women (AGYW) in Uganda despite HIV prevention programs. Rapid testing for recent infections (RTRI) using a commercially-available test can identify areas potentially needing interventions; the addition of viral load testing to RTRI-positive patient samples improves the specificity. We describe results of HIV test positivity and rapid testing for recent infections (RTRI) among AGYW from 2017–2021 to inform targeted planning for additional interventions among AGYW.</p>