<p>Introduction: Botswana is listed among the high burden countries for Tuberculosis (TB) and has one of the highest TB incidence globally (&gt;300/100,000 persons). TB is potentially fatal to humans but curable. The laboratory plays a role in diagnosis, thus facilitating treatment, limiting spread, and monitoring trend. In Southeast District Botswana however, little is known about TB trends as laboratory data has never been analyzed. This study described confirmed cases, determined proportion of Rifampicin Resistant TB (RR-TB) and laboratory turnaround time.</p>