<p>Methods: In a cross-sectional study, four hundred and eighty-six (486) pregnant women attending Papa &amp; Kadiatou (Pa&amp;Ka) medical laboratory in Bamako and five health centres in Ibadan, for routine ante-natal care were enrolled into the study. Antibodies (IgG, IgM) to Toxoplasma gondii and Rubella were assessed by chemiluminescence (Elecsys). Demographics of participants and risk factors of Toxoplasmosis were obtained from questionnaires.</p>