<p>A Descriptive epidemiological study was performed. Cases were identified using the case definition stated as any person from Ruangwa or Kilwa Districts, presenting with fever and bleeding (nasal, from the ear epistaxis, blood in vomitus, sputum, stool or urine) with or without difficulty in breathing, Headache, abdominal pain, diarrhea, and vomiting as well as laboratory confirmed leptospirosis cases or death from 5th July 2022. Blood samples were collected and tested using RNA transcript sequencing using oxford nanopore technology. Analysis of quantitative data was done by using Epi info software version 7.</p>