<p>Japanese encephalitis virus (JEV) is a zoonotic, vector-borne virus spread primarily by Culex mosquitoes, and it causes acute encephalitis in horses and humans. JEV is widely endemic to Southeast Asia and the Western Pacific region but has been reported in Angola. Continuous serosurvey for JEV antibodies in wildlife is important in determining disease distribution and the potential amplifiers. The study determined the factors associated with JEV seropositivity in hunted wildlife from Yamaguchi prefecture.</p>