<p>We analyzed routine HIV testing program data from 2017–2021 for AGYW (aged 10-24 years) reported through the Health Management Information System and RTRI data from the 600 clinical sites that test for HIV recency in Uganda from November 2019–November 2021. Recent infections were defined by positive results using ASANTE rapid kit. We evaluated trends in the positivity rate at the national and district levels and analyzed trends using logistic regression. We calculated the proportion of RTRI-recent HIV infections and identified geographic clusters of recent infections (≥5 RTRI-recent infections in a sub-county within 12 months) among AGYW newly diagnosed with HIV.</p>