<p>Dengue Fever (DF) is caused by a flavivirus called dengue virus (DENV). DF is mainly transmitted through the bite of infected Aedes mosquitoes. Globally recognized as the most important mosquito-transmitted arboviral disease. DF is epidemic-prone and requires immediate reporting, however, no existing DF surveillance or prevalence study had hitherto been conducted in Bayelsa state, Nigeria, despite the vector presence. The aim of this study was to carry out a pilot serological and molecular survey of DF among febrile patients in four referral hospitals in Yenagoa, Bayelsa</p>