<p>We conducted a secondary data analysis of AMR testing data at 7 microbiology laboratories in Zambia collected from 2020 to 2022. Specimens were collected from patients undergoing microbiological investigations for clinical care, and pathogens identification was done using antimicrobial susceptibility testing. We extracted the AMR data from WHONET software, a global platform for surveillance of AMR for laboratory-based surveillance of infectious diseases and AMR. We calculated the proportions of methicillin-resistant Staphylococcus aureus (MRSA), carbapenem-resistant Acinetobacter spp. (CRA), vancomycin-resistant enterococcus spp. (VRE), and extended-spectrum beta-lactamase-resistant organisms (ESBLs) in Zambia.</p>