<p><strong>Methods: </strong>School based cross-sectional study design was conducted from February 09 to March 09,2024 among female secondary school students in Kemissie town, Northeast Ethiopia. A total of 424 female students aged 14 to 19 years were selected using a computer generated simple random sampling technique and participated in the study. Data were collected using structured and self-administered questionnaire and entered into Epi-Info software then exported to SPSS for analysis. Descriptive statistics were analyzed using frequency and crosstab. Multivariable logistic regression analysis was conducted at P value &lt; 0.05 and 95% CI to identify factors significantly associated with Human papillomavirus vaccination uptake.</p>