<p><strong><em>Result:&nbsp;</em></strong><em>Out of the 239 samples processed, 159 were collected from surfaces and 80 from the air. Among these, 85.8% of them were found to have bacterial growth. This resulted in the isolation of 243 bacterial strains, with 89.7% (n=218) Gram-positive and 10.3% (n=25) Gram-negative. CONS (39.5%) and S. aureus (31.3%), were the most dominant isolates. The bacterial load on surfaces and in the air exceeded the standard limits, and the antimicrobial susceptibility analysis revealed that 25.9% of the isolates were resistant to three or more antibiotics. Additionally, 25% of S. aureus and 18.8% of the CONS were methicillin-resistant. From the 25 Gram-negative bacteria, 24% of them were carbapenemase-producing, and 30% ESBL-PE were identified. The biofilm formation assay revealed that 72.3% (34/47) were biofilm producers.</em></p>