<p><strong><em>Methods:</em></strong><em> A cross-sectional study was conducted at JMC (April-August 2022). 239 environmental samples (159 surface, 80 air) were collected using convenient sampling. Settle plates and swabs were used for air and surface sampling, respectively. Standard bacterial culture and biochemical tests identified isolates. Antimicrobial susceptibility was determined using the Kirby-Bauer method. ESBL production was confirmed by the combination disk method, carbapenemase production screened by the Modified Carbapenem Inactivation Method, and MRSA detected using cefoxitin discs. Biofilm formation was assessed using a microtiter plate assay. Data were analyzed using descriptive statistics in SPSS 25.</em></p>